Data Collection

Data Collection

Please acknowledge the BioX core facility (RRID:SCR_019310) in publications, on posters, or in talks if you use any instruments in the SIP core facility. Please include BioX's RRID (RRID:SCR_019310) and the grant numbers for instruments funded through instrumentation grants in your acknowledgements. This is a requirement from the funding agencies and is crucial for future funding. Find example text on theÌýAcknowledgement Page.Ìý

RIGAKU XtaLר MM003

Rigaku MM007 instrument

The XtaLר MM003 is equipped with a MicroMax-003 Microfocus sealed tube X-ray generator system, an AFC11 Partial-χ 4-axis goniometer, a Dectris PILATUS 200K 2D hybrid pixel array detector, and an Oxford cryostream 700. Data collection is managed using HKL 3000, which also allows for indexing, scaling, and integration of the data. The program can suggest data collection strategies based on indexing the initial frames, and it includes a specific strategy program from RIGAKU integrated into HKL 3000. HKL 3000 has automated data processing and structure solution functions, enabling users to go from raw diffraction data to an almost complete protein model with minimal input. Data can also be exported for processing and structure building with various alternative programs.

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Remote Data Collection

We have tools available to ship frozen crystals to synchrotrons for data collection. We are using the Unipuck system. There are two shipping canes holding 7 pucks each, Unipucks (for 16 Xtals each), puck pushers, puck wands, puck separating tools, bent and straight cryo tongs, puck loading dewars and two travel dewars.

Users are expected to organize beam time at synchrotrons and agreements with synchrotrons themselves.

Two workstations are set up in the X-ray facility for remote data collection at the ALS. Other connections can be installed as necessary.

  • Prospective users should contact Dr Erbse if they are interested in using the X-ray facility and to schedule hands-on training. New users are encouraged to discuss their planned crystallisation experiments with Dr Erbse during the planning phase of their work. This will allow them to benefit from her expertise and ensure that their experiments can be conducted using the tools available in BioX.
  • All users must complete the online before they can enter the X-ray facility to receive training from Dr.Erbse.
  • Users must receive hands-on training from Dr Erbse before being allowed to use the XtaLר MM003 independently. This training consists of at least one session and, where possible, uses user samples and lysozyme crystals.
  • After users have complited all online and hands-on training, they will be given access to the Google calendar and the X-ray facility.
  • Users must sign up for instrument time using the Google Calendar.
  • Users must inform Dr. Erbse at least two days in advance if they are planning to use the X-ray generator.
  • All the instruments in the X-ray core are located in the basement of JSCBB in the C-Wing, room C1B51, on East Campus. Card access is required at all times. Users will only be given access after completing both online and in-person trainings
  • Users are expected to provide all consumables specific to their experiment that includes the low pressure liquid nitrogen tank for the cryosystem.Ìý
  • Groups are expected to pay for any parts or repairs due to damage by neglect or carelessness.Ìý
  • Cost for repairs/parts/services resulting from normal wear will be split between all user groups.
  • Please note: ÌýSince repairs/service from normal wear might not be necessary every year, depending on use, these costsÌýmight come quite some time after initial use.
  • Everyone is required to pass the University Ìý beforeÌýusing data collection systems.
  • Everyone must receive hands-on training from Dr. Erbse and be approved to use our data collection systems, before they can do experiments independently.
  • Users have to sign up for time using the google calendars.
  • Users must inform Dr. Erbse of plans to run the X-ray source at least 2 days in advance.
  • Users have to follow all safety regulations at all times. They will learn about essential safety regulations during the different trainings. (See "Safety First" page for more details).
  • Don’t allow unauthorized persons in the X-ray facility!