Shen Lab Selected Publications
(# equal authorship)
Wan, C., Puscher, H., Ouyang, Y., Wu, J., Tian, Y., Li, S., Yin, Q.,Shen, J. (2024) An AAGר-to-CCDC32 handover mechanism controls the assembly of the AP2 adaptor complex. Proc Natl Acad Sci U S A. Aug 20;121(34):e2409341121. PMID: 39145939.
- dzԳٲ:Hollopeter G. (2024) Stepwise assembly of the AP2 endocytic clathrin adaptor complex. Proc Natl Acad Sci U S A. 2024 Sep 17;121(38):e2415313121. PMID: 39250673.
Gaikwad, H., Siegel, D., Barnes, A., Kanada, M., Wan, C.,Shen, J., Balyasnikova, I.V., Simberg, D. (2023) Cyanine lipids promote the shedding of extracellular vesicles from cell membranes. Precision Nanomedicine. Vol 6; Issue 3, 1066-1077.
Wang, S., Chen, X., Crisman, Lauren., Dou, X., Winborn, C.S., Wan, C., Puscher, H., Yin, Q., Kennedy, M.J., and Shen, J. (2023) Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module. Science Advances.Feb 22;9(8):eade2540. PMID: 36812304.
Yu, H., and Shen, J. (2023) Faithful SM proteins chaperone SNAREs on path to successful assembly. Proc Natl Acad Sci U S A. Jan 17;120(3):e2219769120. PMID: 36623199. (Review)
Tian, Y., #, Datta, I. #, Yang, R. #, Wan, C., Wang, B., Crisman, L., He, H., Brautigam, C.A., Li, S., Shen,J., and Yin, Q. (2023) Oligomer-to-monomer transition underlies the chaperone function of AAGר in AP1/AP2 assembly. Proc Natl Acad Sci U S A. Jan 10;120(2):e2205199120. PMID: 36598941. (J. Shen and Y. Qin are both corresponding authors)
Wang, S., Wan, C., Squiers, G.T., Shen, J. (2022) Endocytosis Assays Using Cleavable Fluorescent Dyes. Methods Mol Biol. 2022;2473:181-194. PMID: 35819767.
Wan, C., Stowell, M.H.B., and Shen, J. (2022) Studies of extracellular vesicle-mediated intercellular cargo transfer in the central nervous system – progress and gaps. Communication Biology.Nov 11;5(1):1223. PMID:36369335. (Review)
Wan C.#, Crisman L.#, Wang B, Tian Y, Wang S, Yang R, Datta I, Nomura T, Li S, Yu H, Yin Q, Shen J. (2021) AAGר is an assembly chaperone regulating AP1 and AP2 clathrin adaptors. J Cell Sci. Sep 8:jcs.258587. doi: 10.1242/jcs.258587. PMID: 34494650.
Liu, Y.#, Wan, C.#, Rathore, S.S., Stowell, M.H.B., Yu, H., and Shen, J. (2021) SNARE zippering is suppressed by a conformational constraint that is removed by v-SNARE splitting. Cell Reports.Jan 12;34(2):108611.
Zhang, X., Xu, Q., Zi, Z., Liu, Z., Wan, C., Crisman, L., Shen, J., Liu, X. (2020) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications. Dev Cell. 2020 Dec 1:S1534-5807(20)30883-2.
Dombach, J.L., Quintana, JLJ, Nagy, T.A., Wan, C., Crooks, A.L., Yu, H., Su, C.C., Yu, E.W., Shen, J., Detweiler, C.S. (2020) A small molecule that mitigates bacterial infection disrupts Gram-negative cell membranes and is inhibited by cholesterol and neutral lipids. PLoS Pathog. 2020 Dec. 8;16(12):e1009119.
Menasche, B.L. #, Davis, E.M. #, Wang, S.#, Ouyang, Y., Li, S., Yu, H., Shen, J. (2020) PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes. Science Advances. Nov 27;6(48):eabc3243.
Wang, S., Liu, Y., Crisman, L., Wan, C., Miller, J., Yu, H., Shen, J. (2020) Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis. Traffic. Oct;21(10):636-646. doi: 10.1111/tra.12760. PMID: 32851733.
Yang, C., Chen, F., Ren, P., Lofchy, L., Wan, C., Shen, J., Wang, G., Gaikwad, H., Ponder, J., Jordan C.T., Scheinman, R., Simberg, D. (2020) Delivery of a model lipophilic membrane cargo to bone marrow via cell-derived microparticles. J Control Release. Oct 10;326:324-334.
Zhang, Q., Xu, Y., Lee, J., Jarnik, M., Wu, X., Bonifacino, J.S., Shen, J., Ye, Y. (2020) A myosin-7B-dependent endocytosis pathway mediates cellular entry of α-synuclein fibrils and polycation-bearing cargos. Proc Natl Acad Sci U S A. May 19;117(20):10865-10875. PMID: 32366666.
Rathore, S.S.#, Liu, Y.#, Yu, H., Wan, C., Lee, M., Yin, Q., Stowell, M.H., Shen, J. (2019) Intracellular vesicle fusion requires a membrane-destabilizing peptide located at the juxtamembrane region of the v-SNARE. Cell Reports. (Article). Dec 24;29(13):4583-4592. PMID: 31875562.
Wang, S.#, Crisman, L. #, Miller, J., Datta, I., Gulbranson, D.R., Tian, Y., Yin, Q., Yu, H., and Shen, J., (2019) Inducible Exoc7/Exo70 knockout reveals a critical role of exocyst in insulin-regulated GLUT4 exocytosis. J. Biol. Chem.Nov. 18. PMID: 31740584.
Gulbranson, D.R.#, Crisman, L.#, Lee, M., Ouyang, Y., Menasche, B.L., Demmitt, B.A., Wan, C., Nomura, T., Ye, Y., Yu, H., and Shen, J. (2019) AAGר controls AP2 adaptor assembly in clathrin‐mediated endocytosis. Dev Cell. 50, 436–446. PMID: 31353312.
Yu, H., Crisman, L. Stowell, M.H., and Shen, J. (2019) Functional reconstitution of intracellular vesicle fusion using purified SNAREs and Sec1/Munc18 (SM) proteins.Methods in Molecular Biology. (Review). 1860:237-249. PMID: 30317509.
Shen, C.#, Liu, Y.#, Yu, H., Gulbranson, D.R., Kogut, I., Bilousova, G., Zhang, C., Stowell, M.H., and Shen, J.(2018) The N-peptide binding mode is critical to Munc18-1 function in synaptic exocytosis. J Biol Chem. Oct 1. pii: jbc.RA118.005254. PMID: 30275014.
Yu, H,, Shen, C., Liu, Y., Menasche, B.L., Ouyang, Y., Stowell, M.H., Shen, J. (2018) SNARE zippering requires activation by SNARE-like peptides in Sec1/Munc18 proteins. Proc Natl Acad Sci U S A. Sep 4;115(36):E8421-E8429. PMID: 30127032.
Menasche, B.L., Crisman, L., Gulbranson, D.R., Davis, E.M., Yu, H., and Shen, J., (2018) Fluorescence activated cell sorting (FACS) in genome-wide genetic screening of membrane trafficking. Current Protocols in Cell Biology. (Review). Sep 28:e68. doi: 10.1002/cpcb.68. PMID: 30265447.
Guan, X., Chaffey, P.K. Wei, X. Gulbranson, D.R. Ruan, Y., Wang, X., Li, Y., Ouyang, Y., Chen, L., Zeng, C., Koelsch, T.N., Tran, A.H., Liang, W.*, Shen, J.* and Tan, Z *. (2018)Chemically Precise Glycoengineering Improves Human Insulin.ACS Chemical Biology. 2018 Jan 19;13(1):73-81. PMID: 29090903 (*corresponding authors)
Gulbranson, D.R., Davis, E.M., Demmitt, B.A., Ouyang, Y., Ye, Y., Yu, H., Shen, J. (2017) RרIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.Proc Natl Acad Sci U S A.Sep 26;114(39):E8224-E8233. PMID: 28894007.(Plus article, Highlighted by Faculty of 1000)
Yu, H.,., Gulbranson, D.R., Paine, A., Rathore, S.S., and Shen, J. (2016)Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites.Proc Natl Acad Sci U S A. April 4. PMID: 27044075.
Shen, C.#, Rathore, S.S.#, Yu, H.#, Gulbranson, D.R., Hua, R., Zhang, C., Schoppa, N.E., and Shen, J., (2015) The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis.Nature Communications.6:8852. PMID:26572858
Yu H.#, Rathore, S.S.#, Shen, C., Liu, Y., Ouyang, Y., Stowell, M.H., and Shen, J. (2015) Reconstituting intracellular vesicle fusion reactions: the essential role of macromolecular crowding.J Am Chem Soc.(Article) 137(40):12873-83.
Davis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen J. (2015) Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins. Cell Reports.(Article) Jun 10. pii: S2211-1247 (15) 00552-5.
Zhang C., Lee, S., Peng, Y., Bunker, E., Shen, C., Giaime, E., Shen, JI., Shen, J., Zhou, Z., and Liu, X. (2015) A Chemical Genetic Approach to Probe the Function of PINK1 in Regulating Mitochondrial Dynamics.Cell Research.Mar;25(3):394-7.
Yu, H, Rathore, S.S., Gulbranson, D.R., Shen, J., (2014) The N- and C-terminal domains of tomosyn play distinct roles in SNARE binding and fusion regulation. J Biol Chem.pii: S0960-9822(14)00840-9. PMID: 25063806.
Shen, J. (2013) New tools for old questions - Voices on Directing Traffic into the Future.Developmental Cell.27 (5) 483.
Yu, H., Rathore, S.S., Lopez, J.A., Davis, E.M., James, D.E., Martin, J.L., Shen, J. (2013) Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins.Proc Natl Acad Sci U S A. 110 (35) E3271-E3280. PMID: 23918365. (*PNAS Plus Article)
- Commentary: Shin, Y.K., (2013) Two gigs of Munc18 in membrane fusion.Proc Natl Acad Sci U S A.110 (35) 14116-14117. PMID: 23946422.
Yu, H., Rathore S.S., Shen, J. (2013) Synip Arrests Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (SNARE)-dependent Membrane Fusion as a Selective Target Membrane SNARE-binding Inhibitor.J Biol Chem.Jun 28;288(26):18885-93. PMID: 23665562.
Yu, H., Rathore, S.S., Davis, E. M., Ouyang, Y., and Shen, J. (2013) Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner.Mol Biol Cell.Apr;24(8):1176-84. PMID: 23427263.
Rathore, S.S., Ghosh, N., Ouyang, Y., Shen, J. (2011) Topological restriction of intracellular membrane fusion machinery.Mol Biol Cell,(14) 2612-9. PMID: 21633111
Rathore, S. S.#, Bend, E.G.#, Yu, H., Hammarlund, M., Jorgensen, E.M., Shen, J. (2010) Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE–Sec1/Munc18 membrane fusion complex.Proc Natl Acad Sci U S A.107 (52), 22399–22406. PMID: 21139055. (Cover story and the only feature article in the issue)
- Commentary: Bacaja, T., Panga, Z. P., and Südhof, T.C., (2010) Testing the SNARE/SM Protein Model of Membrane Fusion. Proc Natl Acad Sci U S A. 107 (52) 22365-22366. PMID: 21169506.
Shen, J*, Rathore, S.S., Khandan, L., Rothman, J.E. (2010). SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion.J Cell Biol.190(1):55-63. PMID: 20603329.(* corresponding author)