Bioengineering Core (combined iPS and Transgenic Cores)
University Of 欧美口爆视频 Denver
iPS Cells
Isolation of Primary Fibroblast from Skin Biopsy or other sources
iPS Cell Generation with Sendai and Lenti Viruses
Immunocytochemistry Staining for Pluripotency Markers
QRT-PCR and RT-PCR for Pluripotency Markers
Embryoid Body Formation and Differentiation
Mycoplasma Testing of iPSC Line
Adapting iPSC to Feeder-Free Growth Conditions
Transgenic and Gene Targeting
Gene Targeting in ES Cells
Karyotyping
Removal of floxP or FRT-flanked DNA from the ES cells genome
Blastocyst Injection
Pronuclear Injection
ZFN, TALEN and CRISPR-mediated gene editing in mouse embryos
Cryopreservation of embryos and sperm
In vitro Fertilization
Embryo Rederivation