phage /lab/aaron-whiteley/ en Phage detection by a bacterial NLR-related protein is mediated by DnaJ /lab/aaron-whiteley/2024/06/04/phage-detection-bacterial-nlr-related-protein-mediated-dnaj Phage detection by a bacterial NLR-related protein is mediated by DnaJ Aaron Whiteley Tue, 06/04/2024 - 09:00 Research Articles DnaJ MS2 NACHT NLR antiphage bNACHT bacteria bacteriophage capsid chaperone coat protein innate immunity phage Amy Conte ,  Madison Ruchel ,  Samantha Ridgeway ,  Emily Kibby ,  Toni Nagy ,  Aaron Whiteley

BioRxiv (2024). PubMed PMID: 38895412; PubMed Central PMCID: .

Abstract

Bacteria encode a wide range of antiphage systems and a subset of these proteins are homologous to components of the human innate immune system. Mammalian nucleotide-binding and leucine-rich repeat containing proteins (NLRs) and bacterial NLR-related proteins use a central NACHT domain to link detection of infection with initiation of an antimicrobial response. Bacterial NACHT proteins provide defense against both DNA and RNA phages. Here we determine the mechanism of RNA phage detection by the bacterial NLR-related protein bNACHT25 in E. coli. bNACHT25 was specifically activated by Emesvirus ssRNA phages and analysis of MS2 phage escaper mutants that evaded detection revealed a critical role for Coat Protein (CP). A genetic assay confirmed CP was sufficient to activate bNACHT25 but the two proteins did not directly interact. Instead, we found bNACHT25 requires the host chaperone DnaJ to detect CP. Our data suggest that bNACHT25 detects a wide range of phages by guarding a host cell process rather than binding a specific phage-derived molecule.

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Conte AN, Ridgeway SM, Ruchel ME, Kibby EM, Nagy TA, Whiteley AT. bioRxiv. 2024 Jun 4;. doi: 10.1101/2024.06.04.597415. PubMed PMID: 38895412; PubMed Central PMCID: PMC11185742.

Conte AN, Ridgeway SM, Ruchel ME, Kibby EM, Nagy TA, ➤Whiteley, AT | BioRxiv 2024

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Traditional 0 On White ]]> Tue, 04 Jun 2024 15:00:00 +0000 Aaron Whiteley 197 at /lab/aaron-whiteley Bacterial cGAS-like enzymes produce 2′,3′-cGAMP to activate an ion channel that restricts phage replication /lab/aaron-whiteley/2023/07/26/bacterial-cgas-enzymes-produce-23-cgamp-activate-ion-channel-restricts-phage-replication Bacterial cGAS-like enzymes produce 2′,3′-cGAMP to activate an ion channel that restricts phage replication Anonymous (not verified) Wed, 07/26/2023 - 10:44 Research Articles 2′3′-³¦³Ò´¡²Ñ±Ê CBASS Cap14 SAVED Saf-2TM antiphage signaling biosensor cGAS ion channel ligand-gated phage Uday Tak ,  Peace Holguin-Walth ,  Aaron Whiteley

BioRxiv (2023) PubMed PMID: 37546940; PubMed Central PMCID: PMC10402079.

Abstract

The mammalian innate immune system uses cyclic GMP–AMP synthase (cGAS) to synthesize the cyclic dinucleotide 2′,3′-cGAMP during antiviral and antitumor immune responses. 2′,3′-cGAMP is a nucleotide second messenger that initiates inflammatory signaling by binding to and activating the stimulator of interferon genes (STING) receptor. Bacteria also encode cGAS/DncV-like nucleotidyltransferases (CD-NTases) that produce nucleotide second messengers to initiate antiviral (antiphage) signaling. Bacterial CD-NTases produce a wide range of cyclic oligonucleotides but have not been documented to produce 2′,3′-cGAMP. Here we discovered bacterial CD-NTases that produce 2′,3′-cGAMP to restrict phage replication. Bacterial 2′,3′-cGAMP binds to CD-NTase associated protein 14 (Cap14), a transmembrane protein of unknown function. Using electrophysiology, we show that Cap14 is a chloride-selective ion channel that is activated by 2′,3′-cGAMP binding. Cap14 adopts a modular architecture, with an N-terminal transmembrane domain and a C-terminal nucleotide-binding SAVED domain. Domain-swapping experiments demonstrated the Cap14 transmembrane region could be substituted with a nuclease, thereby generating a biosensor that is selective for 2′,3′-cGAMP. This study reveals that 2′,3′-cGAMP signaling extends beyond metazoa to bacteria. Further, our findings suggest that transmembrane proteins of unknown function in bacterial immune pathways may broadly function as nucleotide-gated ion channels.

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Citation

Tak U, Walth P, Whiteley AT. bioRxiv. 2023 Jul 24;. doi: 10.1101/2023.07.24.550367. PubMed PMID: 37546940; PubMed Central PMCID: PMC10402079.

Tak U, Walth P, ➤Whiteley AT | BioRxiv 2023

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